Production of high-antioxidant yoghurt using phycocyanin from microalgae Spirulina sp

Authors

  • Dhyeta Ulzana Zizi Rahma Diponegoro University image/svg+xml
  • Ghiyats Hafizh Nurahman Diponegoro University image/svg+xml
  • Hadiyanto Hadiyanto Diponegoro University image/svg+xml
  • Rifqi Ahmad Baihaqi Center of Biomass and Renewable Energy (CBIORE)

DOI:

https://doi.org/10.61435/jese.2023.9

Abstract

Yoghurt is a functional food product widely used to improve the digestive system in the body. Yoghurt fermentation usually uses lactic acid bacteria from the Lactobacillus bulgaricus, Streptococcus thermophilus, and Lactobacillus casei. Phycocyanin has been used as a natural dye for food, cosmetics, and medicine. Phycocyanin is a complex protein that can be an anticancer, antioxidant, and immunity booster. In this study, the effect of the number of additions of phycocyanin, storage time, and time of addition of phycocyanin to the antioxidant activity, protein, and organoleptic properties of the yoghurt produced. In this study, cow milk was pasteurized and fermented to become a yoghurt. Afterwards, phycocyanine was added to the yoghurt before being stored and analyzed. Spectrophotometric analysis was utilized to determine the antioxidant activity and protein content. An organoleptic test using a Likert scale was conducted to determine the product's suitability to consumer tastes. Increased phycocyanin concentrations were added (0, 0.25, 0.5, and 0.75 wt. %), resulting in a decrease in IC50 value of 8855.53, 5843.371, and 4147.548 ppm, which shows an increase in antioxidant activity. At the level of consumer preference in terms of taste, the most preferred is a concentration of 0.5 wt. %, while in terms of colour and aroma, at a concentration of 0.25 wt. %. While the longer the storage time (1, 3, and 5 days), the increase in IC50 value of 2777.111, 5179.547, and 5916.884 ppm, and there was a decrease in protein content in the sample. For the level of consumer preferences in terms of taste and aroma, the most preferred is variable with a storage time of 1 day, and in terms of colour, the most preferred at five days storage. The IC50 value in the variable with the addition of phycocyanin after fermentation is less than the addition after fermentation, which is 5351.865 ± 1606 and 3897.162 ± 1678 ppm. The protein content is higher when adding phycocyanin before fermentation. While yoghurt with the addition phycocyanin before fermentation showed higer consumer preference in terms of taste and aroma. In contrast, the addition of phycocyanin after fermentation is preferred in terms of colour. 

Keywords:

Yoghurt, Phycocyanin, Antioxidants, Protein, Organoleptic

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References

Badan Pusat Statistik. (2008). Produksi Yoghurt di Indonesia [Yoghurt Production in Indonesia]. Jakarta: Badan Pusat Statistik.

Barus, P. 2009. Pemanfaatan Bahan Pengawet dan Antioksidan Alami pada Industri Bahan Makanan [Utilization of Natural Preservatives and Antioxidants in the Food Industry]. Medan: Universitas Sumatra Utara.

Bio-Rad Laboratories. (2018). Quick Start Bradford Protein Assay Instruction Manual.

Buckle, K.A., Edwards, R.A., Fleet, G.H., and Wotton, M. (1987). Ilmu Pangan [Food Science]. Penerjemah Hari Purnomo dan Adiono. Jakarta: Universitas Indonesia Press.

Christaki, E., Bonos, E., Florou-Paneri, P. (2015). Innovative Microalgae Pigments as Functional Ingredients in Nutrition. Handbook of Marine Microalgae Biotechnology Advances, 233-241.

Dissanayake, M., Ramchandran, L., Donkor, O.N., dan Vasiljevic, T. (2013). Denaturation of whey proteins as a function of heat, pH and protein concentration. International Dairy Journal, 93-99.

Hamid, A.A., O.O Aiyelaagbe, L.A. Usman, O.M. Ameen, dan A. Lawal. (2010). Antioxidant: its Medical and Pharmacological Applications. African Journal of Pure and Applied Chemistry 4(8):142-151.

Izadi, Z., Nasirpour, A., Garoosi, G.A. & Tamjidi, F. (2015). Rheological and Physical Properties of Yoghurt Enriched with Phytosterol During Storage. Journal of Food Science and Technology, 52 (8), 5341–5346. doi: 10.1007/s13197-014-1593-2

Kozlenko, R, dan Henson, RH. 1998. Spirulina: Effects on the AIDS Virus, Cancer and the Immune System. Spirulina Health Library. www.spirulina.com.

Malik P, Kempanna C, Paul A. (2013). Quality Characteristics of Ice Cream Enriched With Spirulina Powders. International Journal of Food and Nutritional Sciences. 2(1).

Monica, Luzar, L.C. (2011). Efek Warna dalam Dunia Desain dan Periklanan [Color Effects in the World of Design and Advertising]. E-Journal Universitas Binus. 2(2): 1085. doi: 10.21512/humaniora.v2i2.3158

Pace, C.N., Trevino, S., Prabhakaran, E., dan Scholtz, J.M. (2004). Protein Structure, stability, and solubility in water and other solvents. Philosophical Transactions of The Royal Society Biological Sciences, 359(1448),1225-1235. doi: 10.1098/rstb.2004.1500

Patel, A., Pawar, R., Mishra, S., Sonawane, S., Ghosh, P.K. (2004). Kinetic studies on thermal denaturation of C-phycocyanin. Indian Journal of Biochemistry & Biophysics, 41, 254-257. http://nopr.niscpr.res.in/handle/123456789/3719

Putri, Elisa. (2016). Kualitas Susu Sapi Segar Berdasarkan Waktu Penyimpanan [Quality of Fresh Cow's Milk Based on Storage Time]. Chempublish Journal 1(2), 14-20.

Rahmawati, I. S., and Suntornsuk, W. (2016). Effects of Fermentation and Storage on Bioactive Activitiesin Milks and Yoghurts. Procedia Chemistry 18, 53–62. doi:10.1016/j.proche.2016.01.010

Richmond, A. (Ed.). (2004). Handbook of microalgal culture: biotechnology and applied phycology (Vol. 577). Oxford: Blackwell science. p.20-39.

Ridlo, A., Sedjati, S., dan Supriyantini, E. (2015). Aktivitas Antioksidan Fikosianin Dari Spirulina Sp. Menggunakan Metode Transfer Elektron Dengan DPPH (1,1-difenil-2-pikrilhidrazil) [Antioxidant Activity of Phycocyanin From Spirulina Sp. Using the Electron Transfer Method with DPPH (1,1-diphenyl-2-picrylhydrazyl)]. Jurnal Kelautan Tropis. 18(2): 58-63. doi: 10.14710/jkt.v18i2.515

Spolaore, P., Joannis-Cassan, C., Duran, E., Isambert, A., (2006). Commercial applications of microalgae. J. Biosci. Bioeng. 101, 87-96. doi: 10.1263/jbb.101.87

Wahyudi, Marman. (2006). Proses Pembuatan dan Analisis Mutu Yoghurt [Yoghurt Manufacturing Process and Quality Analysis]. Buletin Teknik Pertanian 11(1), 12-16.

Yuguchi, H., T. Goto, dan S. Okonogi. (1992). Fermented Milk, Lactic Drinks, and Intestinal Mikroflora. In: Nakazawa, Y. dan A. Hosono (eds.).(1992). Function of Fermented Milk : Challenge for The Health Science. New York: Elsevier Applied Science.

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Published

2023-12-25

How to Cite

Rahma, D. U. Z., Nurahman, G. H., Hadiyanto, H., & Baihaqi, R. A. (2023). Production of high-antioxidant yoghurt using phycocyanin from microalgae Spirulina sp. Journal of Emerging Science and Engineering, 1(2), 36–43. https://doi.org/10.61435/jese.2023.9

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